Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. JNK1(SAPK1) Mw; 48,000 JNK2(SAPK2) MW; 48,000
HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,SAPK/JNK (別名JNK1/JNK2 あるいはSAPK1/SAPK2)のリン酸化状態の変化を Zn2+-Phos-tag SDS-PAGEで調べています。
c-Jun
JNK1/JNK3
Zn2+ -Phos-tag: 25µM
Polyacrylamide: 8.5%(w/v)
WB:
anti-SAPK/JNK(56G8) CST #9258